[et_pb_section fb_built=”1″ admin_label=”Section” _builder_version=”4.27.5″ _module_preset=”default” custom_margin=”220px||||false|false” custom_padding=”1px|||||” global_colors_info=”{}”][et_pb_row _builder_version=”4.27.5″ _module_preset=”default” custom_margin=”||||false|false” custom_padding=”0px||||false|false” hover_enabled=”0″ global_colors_info=”{}” max_width=”none” sticky_enabled=”0″][et_pb_column type=”4_4″ _builder_version=”4.21.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.5″ _module_preset=”default” custom_margin=”||||false|false” global_colors_info=”{}”]<\/p>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row admin_label=”Row” _builder_version=”4.21.0″ _module_preset=”default” global_colors_info=”{}” column_structure=”1_2,1_2″ custom_margin=”||||false|false” custom_padding=”0px||||false|false” max_width=”none”][et_pb_column type=”1_2″ _builder_version=”4.21.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.0″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n
The tuberculosis Molecular Bacterial Load Assay (TB-MBLA) test is the first of its kind in the world of mycobacteriology. The test deploys molecular principles to detect and quantify viable Mycobacterium tuberculosis<\/em> (Mtb) load in a patient sample. Ability to detect viable bacteria enables the use of the test for monitoring response to anti-TB therapy, in which case it measures the number of live bacteria left following initiation of treatment.<\/p>\n The translational training is aimed at clinical and laboratory practitioners and researchers. The training will cover the technical aspects of performing TB-MBLA test as well as contextual aspects of applying the test in the real-world clinical setting. Technical aspects will include isolation of RNA from Mtb, preparation and running of reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) and analysis of the RT-qPCR results. Translational aspects will include interactive discussion on interpretation of results in a clinical context, and on where and when to deploy the test in a routine clinical setting. The last session will cover the principles of translating biomedical research innovations into policy and practice.<\/p>\n Map showing the growing coverage of TB-MBLA as of August 2024.<\/em><\/strong><\/p>\n [\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.21.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”https:\/\/medicine.st-andrews.ac.uk\/tbtrials\/wp-content\/uploads\/sites\/50\/2024\/08\/TBMBLA-Heat-Map.png” title_text=”TBMBLA Heat Map” admin_label=”TBMBLA Heat Map” _builder_version=”4.27.5″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” force_fullwidth=”on” sticky_enabled=”0″][\/et_pb_image][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.27.5″ _module_preset=”default” custom_margin=”||||false|false” custom_padding=”0px||||false|false” max_width=”none”][et_pb_column _builder_version=”4.27.5″ _module_preset=”default” type=”4_4″][et_pb_divider _builder_version=”4.27.0″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_divider][et_pb_heading title=”Diagram Showing TBMBLA Workflow” admin_label=”Heading” _builder_version=”4.27.0″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_heading][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.27.5″ _module_preset=”default” column_structure=”1_2,1_2″ custom_margin=”||||false|false” custom_padding=”0px||||false|false” max_width=”none”][et_pb_column _builder_version=”4.27.5″ _module_preset=”default” type=”1_2″][et_pb_image src=”https:\/\/medicine.st-andrews.ac.uk\/tbtrials\/wp-content\/uploads\/sites\/50\/2024\/08\/TBMBLA-Workflow.png” title_text=”TBMBLA Workflow” admin_label=”TB-MBLA The Workflow Image” _builder_version=”4.27.5″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” force_fullwidth=”on” sticky_enabled=”0″][\/et_pb_image][\/et_pb_column][et_pb_column _builder_version=”4.27.5″ _module_preset=”default” type=”1_2″][et_pb_text admin_label=”Text” _builder_version=”4.27.0″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n Diagram overview of the Molecular Bacterial Load Assay (MBLA) process:<\/strong><\/p>\n Figure 1. <\/b>\u00a0Patient sample collection.<\/p>\n Figure 2. <\/b>Spiking in of a known concentration of the extraction control into the patient sample and RNA extraction.<\/p>\n Figure 3. <\/b>RT-qPCR using two sets of oligo, each with a different fluorophore.<\/p>\n Figure 4. <\/b>Analysis of quantification in two separate channels, Green channel for sample quantification, Yellow channel for extraction control quantification.<\/p>\n [\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":" TB-MBLAThe tuberculosis Molecular Bacterial Load Assay (TB-MBLA) test is the first of its kind in the world of mycobacteriology. The test deploys molecular principles to detect and quantify viable Mycobacterium tuberculosis (Mtb) load in a patient sample. Ability to detect viable bacteria enables the use of the test for monitoring response to anti-TB therapy, in […]<\/p>\n","protected":false},"author":159,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-6160","page","type-page","status-publish","hentry"],"publishpress_future_action":{"enabled":false,"date":"2026-04-18 00:02:47","action":"change-status","newStatus":"draft","terms":[],"taxonomy":"","extraData":[]},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/medicine.st-andrews.ac.uk\/tbtrials\/wp-json\/wp\/v2\/pages\/6160","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/medicine.st-andrews.ac.uk\/tbtrials\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/medicine.st-andrews.ac.uk\/tbtrials\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/medicine.st-andrews.ac.uk\/tbtrials\/wp-json\/wp\/v2\/users\/159"}],"replies":[{"embeddable":true,"href":"https:\/\/medicine.st-andrews.ac.uk\/tbtrials\/wp-json\/wp\/v2\/comments?post=6160"}],"version-history":[{"count":4,"href":"https:\/\/medicine.st-andrews.ac.uk\/tbtrials\/wp-json\/wp\/v2\/pages\/6160\/revisions"}],"predecessor-version":[{"id":7016,"href":"https:\/\/medicine.st-andrews.ac.uk\/tbtrials\/wp-json\/wp\/v2\/pages\/6160\/revisions\/7016"}],"wp:attachment":[{"href":"https:\/\/medicine.st-andrews.ac.uk\/tbtrials\/wp-json\/wp\/v2\/media?parent=6160"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}