{"id":501,"date":"2021-03-02T14:36:48","date_gmt":"2021-03-02T14:36:48","guid":{"rendered":"http:\/\/med.st-andrews.ac.uk\/staar\/?page_id=501"},"modified":"2021-03-02T14:36:48","modified_gmt":"2021-03-02T14:36:48","slug":"tb-mbla","status":"publish","type":"page","link":"https:\/\/medicine.st-andrews.ac.uk\/staahr\/education-and-training\/tb-mbla\/","title":{"rendered":"Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)"},"content":{"rendered":"

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Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)<\/h1>\n

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[\/et_pb_text][et_pb_text admin_label=”Training Intro” _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”]The tuberculosis Molecular Bacterial Load Assay (TB-MBLA) test is the first of its kind in the world of mycobacteriology. The test deploys molecular principles to detect and quantify viable Mycobacterium tuberculosis<\/em> (Mtb) load in a patient sample. Ability to detect viable bacteria enables the use of the test for monitoring response to anti-TB therapy, in which case it measures the number of live bacteria left following initiation of treatment.<\/p>\n

The translational training is aimed at clinical and laboratory practitioners and researchers. The training will cover the technical aspects of performing TB-MBLA test as well as contextual aspects of applying the test in the real-world clinical setting. Technical aspects will include isolation of RNA from Mtb, preparation and running of reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) and analysis of the RT-qPCR results. Translational aspects will include interactive discussion on interpretation of results in a clinical context, and on where and when to deploy the test in a routine clinical setting. The last session will cover the principles of translating biomedical research innovations into policy and practice.
\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_4,3_4″ _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_4″ _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”https:\/\/medicine.st-andrews.ac.uk\/staahr\/wp-content\/uploads\/sites\/49\/2021\/03\/20210311-JoVELogo.png” alt=”Jove Logo” admin_label=”Jove Logo” _builder_version=”4.9.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][et_pb_column type=”3_4″ _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_text admin_label=”TB-MBLA Published in JoVE” _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”]Link describes the TB-MBLA processes. It describes the process from RNA extraction to PCR and results. . Read more.<\/a>
\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”]Spiking the extraction control into the sample is crucial to control for extraction and downstream PCR processes. This processes is explained in further details in the below videos:
\n[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_3,2_3″ _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_text admin_label=”Video 1 Intro” _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”]This introduces the clinical scenario and explains the clinical implication that warrants performance of TB-MBLA. Next the video explains the important steps of RNA extraction, PCR prep and results interpretation.
\n[\/et_pb_text][\/et_pb_column][et_pb_column type=”2_3″ _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_video src=”https:\/\/www.youtube.com\/watch?v=VBmmSzpZhCA&list=PLkZBIZv_vTvnf7ZvIye6GTV332Y5lhMcV&index=1″ admin_label=”TB-MBLA Intro” _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_video][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_3,2_3″ _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_text admin_label=”Video 2 Intro” _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”]Describes the PCR results analysis including the importation and use of standard curve to translate quantification cycles (Cq) into bacterial load (CFU\/mL).
\n[\/et_pb_text][\/et_pb_column][et_pb_column type=”2_3″ _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_video src=”http:\/\/www.youtube.com\/watch?v=OXfmOSOr4mA&list=PLkZBIZv_vTvnf7ZvIye6GTV332Y5lhMcV&index=2″ admin_label=”TB-TBLA PCR Results Analysis” _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_video][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_3,2_3″ _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_text admin_label=”Video 3 Intro” _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”]Recaps PCR results analysis and explains the clinical implications of such results, It invites students to think about further investigations they may have to do in order understand the patient and their surrounding.
\n[\/et_pb_text][\/et_pb_column][et_pb_column type=”2_3″ _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_video src=”https:\/\/www.youtube.com\/watch?v=MoT5StOrsD0&list=PLkZBIZv_vTvnf7ZvIye6GTV332Y5lhMcV&index=3″ admin_label=”TB-MBLA Results in Clinical Context” _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_video][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_3,2_3″ _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column][et_pb_column type=”2_3″ _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][et_pb_button button_url=”https:\/\/medicine.st-andrews.ac.uk\/staahr\/education-and-training\/tb-mbla\/20210317-tb-mbla\/” button_text=”17 March 2021 Training” admin_label=”20210317 Training ” _builder_version=”4.9.0″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_button][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.9.10″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.9.10″ _module_preset=”default” global_colors_info=”{}”][et_pb_divider color=”#E09900″ _builder_version=”4.9.10″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_3,2_3″ _builder_version=”4.9.4″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.9.4″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”https:\/\/medicine.st-andrews.ac.uk\/staahr\/wp-content\/uploads\/sites\/49\/2021\/04\/20210405-VitalBacteria.png” alt=”Vital Bacteria Logo” url=”https:\/\/www.vitalbacteria.com\/” admin_label=”Vital Bacteria Logo” _builder_version=”4.9.4″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][et_pb_column type=”2_3″ _builder_version=”4.9.4″ _module_preset=”default” global_colors_info=”{}”][et_pb_text admin_label=”Vital Bacteria Text” _builder_version=”4.9.4″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n

The University of St Andrews School of Medicine leads in manufacturing the TB-MBLA kits under the trademark\u00a0Vitalbacteria<\/a>. The kits are distributed by the SOI Group Ltd.<\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_3,2_3″ _builder_version=”4.9.10″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.9.10″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.9.10″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n

Scientists at the University of St Andrews have developed an innovative test to measure tuberculosis bacteria and how they change in response to medication in 4h. The test compromises three steps: 1) isolation of Mycobaterium tuberculosis RNA from the patient sample, 2) Reverse transcriptase quantitatve polymerase chain reaction (RT-qPCR) and 3) translation of the RT-qPCR output to bacterial load measured as colony forming units per mL.<\/p>\n

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Tuberculosis Molecular Bacterial Load Assay (TB-MBLA) The tuberculosis Molecular Bacterial Load Assay (TB-MBLA) test is the first of its kind in the world of mycobacteriology. The test deploys molecular principles to detect and quantify viable Mycobacterium tuberculosis (Mtb) load in a patient sample. Ability to detect viable bacteria enables the use of the test for […]<\/p>\n","protected":false},"author":126,"featured_media":0,"parent":61,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-501","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/medicine.st-andrews.ac.uk\/staahr\/wp-json\/wp\/v2\/pages\/501","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/medicine.st-andrews.ac.uk\/staahr\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/medicine.st-andrews.ac.uk\/staahr\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/medicine.st-andrews.ac.uk\/staahr\/wp-json\/wp\/v2\/users\/126"}],"replies":[{"embeddable":true,"href":"https:\/\/medicine.st-andrews.ac.uk\/staahr\/wp-json\/wp\/v2\/comments?post=501"}],"version-history":[{"count":0,"href":"https:\/\/medicine.st-andrews.ac.uk\/staahr\/wp-json\/wp\/v2\/pages\/501\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/medicine.st-andrews.ac.uk\/staahr\/wp-json\/wp\/v2\/pages\/61"}],"wp:attachment":[{"href":"https:\/\/medicine.st-andrews.ac.uk\/staahr\/wp-json\/wp\/v2\/media?parent=501"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}