Dr Gordon Cramb

Dr Gordon Cramb
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Departmental Duties:

  • School Safety Officer (Research)
  • Module controller MD3002
data source: symbiosis

Research Overview from research@st-andrews

Osmoregulation is key to the survival of all organisms, from unicellular bacteria and archae to multicellular plants and animals, including mammals and man. The physiological problems faced by any organism depend on the composition of the immediate external environment and the intracellular ion and solute composition required for normal cell viability, general health, growth and successful reproduction.  All organisms, whether unicellular or multicellular, have to sustain an intracellular ion and solute composition distinct from that of the extracellular fluid, whilst sustaining water homeostasis.  In multicellular organisms, the maintenance of this dis-equilibrium of solutes across biological membranes is mediated by the activity of a variety of ion, water and solute transporters, which are often selectively expressed and located within different membrane compartments of polarized epithelial cells.  The expression, location and function of these membrane transport proteins can often be regulated, both acutely and chronically, by a myriad of extracellular signaling molecules including hormones and neurotransmitters.  Studies in my laboratory have mainly focused on investigations of the functions of many transporters, channels and enzymes expressed within the epithelial tissues of euryhaline fish, which have been selected as models for the study of the control of ion and water transport across biological membranes.  These investigations have highlighted the functional roles of many genes in many tissues and their role in successful osmoregulation when euryhaline fish move between environments of different salinity (ie freshwater to seawater).  Studies have also highlighted a role for members of the gut peptide family, called guanylins, and their actions, both locally in the GI tract as well as in the kidney, have been investigated.  The peptides mediate their response by binding and activating an integrate membrane guanylate cyclase (type C) with concomitant increases in intracellular cyclic GMP.   An additional, and novel protein involved in as yet unknown signaling pathways within epithelial cells in the eel gut, and which has a role in protein expression, has also been identified in mammalian cells and tissues.  These signaling molecules, which are members of the phospholipase C super-family, are currently being investigated at the molecular levels in cells and tissues from both teleost and mammalian origins.


Recent publications listed in research@st-andrews
25 (of 68 published available) for gc (source: University of St Andrews PURE)
Please click title of any item for full details

Myo-inositol phosphate synthase expression in the European eel (Anguilla anguilla) and Nile tilapia (Oreochromis niloticus) Svetlana Kalujnaia, Neil Hazon, Gordon Cramb
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology 2016 vol.311 pp.R287-R298
In absence of local adaptation, plasticity and spatially varying selection rule Caroline L. Cote, Martin Castonguay, McWilliam Svetlana Kalujnaia, Gordon Cramb, Louis Bernatchez
BMC Genomics 2014 vol.15 
Seawater acclimation and inositol monophosphatase isoform expression in the European eel (Anguilla anguilla) and Nile tilapia (Orechromis niloticus) Svetlana Kalujnaia, Neil Hazon, Alfredo Villasenor, Paul H. Yancey, Gordon Cramb
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology 2013 vol.305 pp.R369-R384
Aquaporin 4 is a ubiquitously expressed isoform in the dogfish (Squalus acanthias) shark Christopher Paul Cutler, Bryce McIver, Gordon Cramb, Mark Zeidel
Frontiers in Physiology 2012 vol.2 
Cloning, tissue distribution and sub-cellular localisation of phospholipase C X-domain containing protein (PLCXD) isoforms Svetlana Kalujnaia, Gordon Cramb
Biochemical and Biophysical Research Communications 2012 vol.424 pp.651-656
Surviving in a toxic world Jose Martin Pujolar, Ilaria A M Marino, Massimo Milan, Alessandro Coppe, Gregory E Maes, Fabrizio Capoccioni, Eleonora Ciccotti, Lieven Bervoets, Adrian Covaci, Claude Belpaire, Gordon Cramb, Tomaso Patarnello, Luca Bargelloni, Stefania Bortoluzzi, Lorenzo Zane
BMC Genomics 2012 vol.13 
Differential timing of gene expression regulation between leptocephali of the two Anguilla eel species in the Sargasso Sea Louis Bernatchez, Jerome St-Cyr, Eric Normandeau, Gregory Maes, Thomas Als, Svetlana Kalujnaia, Gordon Cramb, Martin Castonguay, Michael Hansen
Ecology and Evolution 2011 vol.1 pp.459-467
Expression and localization of Aquaporin 1a in the sea-bass (Dicentrarchus labrax) during ontogeny Ivone Giffard-Mena, Viviane Boulo, Charline Abed, Gordon Cramb, Guy Charmantier
Frontiers in Physiology 2011 vol.2 
Phospholipase C-η2 is activated by elevated intracellular Ca2+ levels. William Beswick, Simon Guild, Gordon Cramb, Kevin Morgan, Robert P. Millar, Alan J. Stewart
Cellular Signalling 2011 vol.23 pp.1777-1784
A role for inositol monophosphatase 1 (IMPA1) in salinity adaptation in the euryhaline eel (Anguilla anguilla) Svetlana Kalujnaia, Jill McVee, Taciana Kasciukovic, Alan J. Stewart, Gordon Cramb
FASEB Journal 2010 vol.24 pp.3981-3991
Identification of Functionally Segregated Sarcoplasmic Reticulum Calcium Stores in Pulmonary Arterial Smooth Muscle Jill H. Clark, Nicholas Patrick Kinnear, Svetlana Kalujnaia, Gordon Cramb, Sidney Fleischer, Loice H. Jeyakumar, Frank Wuytack, A. Mark Evans
Journal of Biological Chemistry 2010 vol.285 pp.13542-13549
Aquaporin 8 (AQP8) intestinal mRNA expression increases in response to salinity acclimation in yellow and silver European eels (Anguilla anguilla) Christopher P. Cutler, Clare Philips, Neil Hazon, Gordon Cramb
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 2009 vol.153A pp.S78
Guanylin-like peptides, guanylate cyclase and osmoregulation in the European eel (Anguilla anguilla) Svetlana Kalujnaia, Gillian Dawn Wilson, Gordon Cramb
General and Comparative Endocrinology 2009 vol.161 pp.103-114
Immunohistochemical localization and expression of aquaporin water channel membrane transport protein homologues in the osmoregulatory tissues of the dogfish (Squalus acanthias) Christopher P. Cutler, Gordon Cramb
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 2009 vol.153A pp.S65-S66
Intestinal expression of Aquaporins in the European eel (Anguilla anguilla): Effects of SW acclimation on isoform expression Gordon Cramb, Brian Powell, Caroline J. Osborne, Anne-Sophie Martinez, Christopher P. Cutler, Svetlana Kalujnaia
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 2009 vol.153A pp.S78
Osmolytes and osmoregulation in the euryhaline European eel, Anguilla anguilla Svetlana Kalujnaia, Caroline J. Osborne, Gordon Cramb
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 2009 vol.153A 
Differential expression of absorptive cation-chloride-cotransporters in the intestinal and renal tissues of the European eel (Anguilla anguilla) Christopher Paul Cutler, Gordon Cramb
Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology 2008 vol.149 pp.63-73
Identification of differential gene expression in the European eel (Anguilla anguilla) following "silvering" Svetlana Kalujnaia, Gordon Cramb, V. Zaguinaiko
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 2008 vol.150 
The role of the gut in hydromineral balance during the ontogeny of the sea bass Dicentrarchus labrax G. Charmantier, I. Giffard Mena, C. Abed, V. Boulo, C. Bodinier, E. Sucre, C. Lorin-Nebel, R. Castille, P. Cucchi-Mouillot, Gordon Cramb
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 2008 vol.150 
A transcriptomic approach to the study of osmoregulation in European eel Anguilla anguilla S Kalujnaia, IS McWilliam, VA Zaguinaiko, AL Feilen, J Nicholson, Neil Hazon, Christopher Paul Cutler, Gordon Cramb
Physiological Genomics 2007 vol.31 pp.385-401
Aquaporin molecular characterization in the sea bass (Dicentrarchus labrax): The effect of salinity on AQP1 and AQP3 expression I Giffard-Mena, V Boulo, F Aujoulat, H Fowden, R Castille, G Charmantier, Gordon Cramb
Comparative Biochemistry and Physiology 2007 vol.149A pp.430-444
Cloning and expression of aquaporin water channels in the euryhaline bull shark, Carcharhinus leucas L. Meischke, Gordon Cramb, Christopher Paul Cutler
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 2007 vol.146 
Cortisol regulates eel (Anguilla anguilla) aquaporin 3 (AQP3) mRNA expression levels in gill Christopher Paul Cutler, C. Phillips, Neil Hazon, Gordon Cramb
General and Comparative Endocrinology 2007 vol.152 pp.310-313
Cortisol regulates eel (Anguilla anguilla) aquaporin 3 (AQP3) mRNA expression levels in gill. Christopher Paul Cutler, C Phillips, Neil Hazon, Gordon Cramb
Comparative Biochemistry and Physiology 2007